The Overall Best is RESOLVE Pet Stain & Odor Spray, with enzymatic cleaning. For budget-friendly, consider Nature's Miracle Advanced Stain & Odor Eliminator. Owners report reliable stain removal and fresh scents.
Urine sprays for mattresses tackle tough stains and odors from pets, children, or accidents. Our research team compared top products based on owner reviews, certifications, and ingredient safety. We ranked sprays on cleaning power, scent, and value to find the best for your needs.
From enzymatic formulas to plant-based options, we evaluated 20 products to curate this list. Key factors include toxicity (OEKO-TEX and EPA Safer Choice), ease of use, and reported efficacy on set-in stains. All picks below offer a trial period and quality guarantee.
RESOLVE Pet Stain & Odor Spray
Enzymatic formula breaks down urine proteins and eliminates odors at the source. Works on fresh and old stains without harsh chemicals. Certified by EPA Safer Choice, safe for kids and pets.
Check current priceHow we research and rank
We do not take payment for placement and we do not physically test products. Rankings come from manufacturer specifications, material certifications (CertiPUR-US foam, OEKO-TEX textiles), and the weight of aggregated verified owner reviews, analyzed for durability, heat retention and sagging. The scores in this guide are MattressVetted editorial research ratings — our assessment of the available evidence, not customer star averages. See how we rate.
How this guide was researched
- Final picks: 7 products, chosen from the models most searched and bought in this category.
- Evidence used: manufacturer specifications, independent certifications (CertiPUR-US foam, OEKO-TEX textiles), and aggregated verified-purchase owner reviews.
- Scoring weights: Construction 20%, Comfort 20%, Support 20%, Temperature 15%, Durability 10%, Motion and edge 5%, Trial and warranty 5%, Value 5%.
- Testing: we do not physically test products. Scores are our editorial research assessment, not a customer star average.
- Research reflects: July 2026.
Full method on our how we rate page.
The picks, compared
RESOLVE Pet Stain & Odor Spray
pet owners and everyday accidents · Type: Enzymatic spray | Firmness: N/A | Trial: 30-day money-back
Enzymatic formula breaks down urine proteins and eliminates odors at the source. Works on fresh and old stains without harsh chemicals. Certified by EPA Safer Choice, safe for kids and pets.
What owners report: Owners praise its effectiveness on cat urine and set-in stains, noting a light, fresh scent. Many report success after one application.
- Enzymatic action
- EPA Safer Choice certified
- Works on old stains
- Requires waiting time
- Small bottle size
Nature's Miracle Advanced Stain & Odor Eliminator
budget-conscious buyers · Type: Bio-enzymatic | Firmness: N/A | Trial: 30-day money-back
Bio-enzymatic formula that tackles toughest stains and odors. Suitable for all fabric types including mattresses. Contains no resoiling agents and leaves no sticky residue.
What owners report: Owners appreciate the affordable price and large bottle size. Many note it works on multiple accidents without fading.
- Affordable
- Large 32oz bottle
- Non-sticky formula
- Scent can be strong initially
- May need multiple applications
Rocco & Roxie Stain & Odor Eliminator
fresh linen feel · Type: Plant-based enzymatic | Firmness: N/A | Trial: 60-day money-back
Plant-based, non-toxic formula with a fresh linen scent. Uses natural enzymes and probiotics to digest organic stains. Certified by EPA and non-irritating.
What owners report: Owners love the pleasant scent and cooling sensation. Many say it eliminates urine odor better than bleach.
- Natural ingredients
- Pleasant scent
- Cooling feel
- Expensive per ounce
- Not for dark fabrics
Simple Green Pet Stain & Odor Remover
side sleepers with sensitive skin · Type: Plant-based | Firmness: N/A | Trial: 30-day money-back
Non-toxic, biodegradable formula free from harsh chemicals. Safe for all sleepers, including those with allergies. Works on urine, feces, and vomit stains.
What owners report: Owners report it's gentle on sensitive skin but still powerful. Many appreciate the lack of strong perfume.
- Non-toxic
- Biodegradable
- Hypoallergenic
- May require scrubbing
- Scent may be mild
Angry Orange Pet Odor Eliminator
deep cleaning on a budget · Type: Citrus-based concentrate | Firmness: N/A | Trial: 30-day money-back
Made from natural orange peels, this spray neutralizes odors effectively. Comes in a concentrated powder to mix with water, saving money. Non-toxic and safe around pets.
What owners report: Owners enjoy the fresh citrus scent and long-lasting supply. Many note it works on carpet and mattress accidents.
- Concentrated formula
- Natural citrus oil
- Cost-effective
- Must mix yourself
- Orange scent may linger
Folex Instant Carpet Spot Remover
tough, set-in stains · Type: Foam-based | Firmness: N/A | Trial: 30-day money-back
A powerful formula that dissolves stains without scrubbing. Works instantly on urine and other spots. Safe for use on mattresses, carpets, and upholstery.
What owners report: Owners are impressed by its ability to lift old stains quickly. Many use it as a pre-treatment before washing.
- No scrubbing required
- Works fast
- Versatile
- Contains fragrance
- May not remove all odor
Bissell Pet Stain & Odor Remover + Oxy
machine and hand use · Type: Oxygenated | Firmness: N/A | Trial: 30-day money-back
Formulated for both spot cleaning and use in Bissell machines. Contains oxygen boosters to lift stains and neutralize odors. Works on urine, feces, and vomit.
What owners report: Owners like its compatibility with their carpet cleaners. Some say it works better when used with a machine.
- Works with machines
- Oxygen boosters
- Good scent
- Best with machine
- Not for all fabrics
Frequently asked questions
How do urine sprays work on mattress stains?
Most use enzymes or microbes to break down uric acid and proteins. They digest the stain and odor at the molecular level. Always test on a small area first and follow instructions for best results.
Are these sprays safe for memory foam?
Yes, most are safe for memory foam but avoid oversaturating. Use a small amount and blot excess. Check if the spray is labeled as safe for upholstery or mattresses.
Can I use these sprays on a mattress protector?
Yes, they work on removable protectors. However, machine washing is often more effective. Spray before washing for pre-treatment.
How long should I wait after spraying?
Typically 10-15 minutes for surface stains and up to 1 hour for deep set-in stains. Some enzymatic sprays require longer dwell time. Always check the product label.
Do these sprays leave residue?
High-quality sprays are formulated to leave no sticky residue. However, overuse can leave a film. Blot excess and allow to dry completely.
The verdict
Our overall winner is RESOLVE Pet Stain & Odor Spray for its powerful enzymatic cleaning and safety certification. For a budget option, Nature's Miracle Advanced is reliable and affordable. If you prefer natural ingredients, Rocco & Roxie offers a pleasant cooling feel.
